Personalized bioconversion of Panax notoginseng saponins mediated by gut microbiota between two different diet-pattern healthy subjects

Abstract Background Panax notoginseng saponins (PNS) as the main effective substances from P. with low bioavailability could be bio-converted by human gut microbiota. In our previous study, PNS metabolic variations mediated microbiota have been observed between high fat, protein (HF-HP) and plant fiber-rich (LF-PF) dietary subjects. this we aimed to correspondingly characterize relationship distinct microbial species metabolites. Methods Gut were collected HF-HP LF-PF healthy adults profiled 16S rRNA gene sequencing. incubated in vitro. A LC–MS/MS method was developed quantify five metabolites yields including ginsenoside F 1 (GF ), Rh 2 (GRh compound K (GC-K), protopanaxatriol (PPT) protopanaxadiol (PPD). The selected species, Bifidobacterium adolescentis Lactobacillus rhamnosus , employed metabolize for corresponding Results significantly different two diet groups. Compared group, genus Blautia Bifidobacterium, Clostridium, Corynebacterium, Dorea, Enhydrobacter Lactobacillus, Roseburia Ruminococcus, SMB53, Streptococcus, Treponema Weissella enriched while Phascolarctobacterium Oscillospira relatively decreased. Furthermore, Spearman’s correlative analysis revealed microbials groups positively negatively associated metabolites, respectively. Conclusions Our data showed diversity led personalized bioconversion of PNS. Graphic